Mouse models to study natural killer cell-mediated immunosurveillance and metastatic latency

Vidhya R. Nair, Srinivas Malladi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Metastatic latency is a major concern in the clinic, yet how these disseminated cancer cells survive and initiate metastases is unknown (Massagué and Obenauf, Nature 529:298–306, 2016). Here, we describe an approach to isolate latency competent cancer (LCC) cells from early stage human lung and breast carcinoma cell lines using mouse xenograft models (Malladi, Cell 165:45–60, 2016). Cancer cell lines labeled with GFP-luciferase and antibiotic selection markers were injected intracardially into athymic mice. Three months, post-injection, LCC cells were identified in situ and isolated. Upon reinjection, LCC cells retain their tumorigenic potential, enter a slow-cycling or quiescent state, and evade NK cell-mediated innate immune surveillance.

LanguageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages141-150
Number of pages10
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1884
ISSN (Print)1064-3745

Fingerprint

Immunologic Monitoring
Natural Killer Cells
Neoplasms
Cell Line
Luciferases
Heterografts
Nude Mice
Breast Neoplasms
Neoplasm Metastasis
Anti-Bacterial Agents
Lung
Injections

Keywords

  • Cancer
  • Disseminated tumor cells
  • Dormancy
  • LCC cells
  • Metastasis
  • Metastatic latency
  • NK cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nair, V. R., & Malladi, S. (2019). Mouse models to study natural killer cell-mediated immunosurveillance and metastatic latency. In Methods in Molecular Biology (pp. 141-150). (Methods in Molecular Biology; Vol. 1884). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8885-3_9

Mouse models to study natural killer cell-mediated immunosurveillance and metastatic latency. / Nair, Vidhya R.; Malladi, Srinivas.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 141-150 (Methods in Molecular Biology; Vol. 1884).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nair, VR & Malladi, S 2019, Mouse models to study natural killer cell-mediated immunosurveillance and metastatic latency. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1884, Humana Press Inc., pp. 141-150. https://doi.org/10.1007/978-1-4939-8885-3_9
Nair VR, Malladi S. Mouse models to study natural killer cell-mediated immunosurveillance and metastatic latency. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 141-150. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8885-3_9
Nair, Vidhya R. ; Malladi, Srinivas. / Mouse models to study natural killer cell-mediated immunosurveillance and metastatic latency. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 141-150 (Methods in Molecular Biology).
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